Journal: PLoS ONE
Article Title: Combining Next-Generation Sequencing and Microarray Technology into a Transcriptomics Approach for the Non-Model Organism Chironomus riparius
doi: 10.1371/journal.pone.0048096
Figure Lengend Snippet: Distribution of the best blastx hits that were matched to the isotigs (black) and the singletons (light grey) according to their taxonomic origin. (A) All transcripts (isotigs n = 16,824; singletons n = 24,129) that were matched to a BLASTX hit. (B) Transcripts (isotigs n = 16,537; singletons n = 4,7539) that were matched to a BLASTX hit and that are targeted by the final aGE microarray.
Article Snippet: The designed 1×1 M probe-selection microarray was obtained from Agilent Technologies and hybridized with C. riparius and A. gambiae genomic DNA (gDNA). gDNA was extracted from 30 pooled fertilized C. riparius egg ropes, respectively, 30 pooled unfed A. gambiae adults.
Techniques: Microarray